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. 2016 May 13;5(7):e1180485. doi: 10.1080/2162402X.2016.1180485

Figure 2.

Figure 2.

TRAP-induced IL-10-producing B cells suppress T-cell proliferation. (A–D) CFSE-labeled purified CD4+ T cells (A and B) and CD8+ T cells (C and D) were stimulated with plate-bound anti-CD3/anti-CD28 mAb and were either cultured alone (T cells only) or co-cultured with TRAP-induced B cells (3 μg/mL) from WT mice or IL-10−/− mice at ratio of 1:1 for 4 d, un-stimulated T cells were used as control. In the transwell experiment, B cells and T cells were added in the upper and lower chambers, respectively. CFSE dilution was determined by flow cytometry. (E–F) CFSE-labeled splenocytes from naive OT-1 mice were stimulated with OVA257–264 (1 µg/mL) and co-cultured with purified B cells, OVA+ TRAP-induced B cells, or OVA− TRAP-induced B cells at ratio of 1:1. Cells were collected after 4 d, stained with anti-CD8+ mAb and anti-Vβ 5.1/5.2. CFSE dilution was determined by flow cytometry. Results are representative of three independent experiments. NS: p > 0.05, *** p < 0.001 by unpaired t-test (B, D and F).