Figure 5.

IL12 enabled DNAM-1 mediated cytotoxicity is dependent on CD45 phosphatase activity. (A) Left: Change in DNAM-1 MFI mediated by IL12. Summary from different T-cell populations (early central memory T cells (Melan A), effector memory T cells (clonal STEAP1-sp. and PRAME-sp. Populations)). Results are from more than 10 experiments. Right: representative histogram of DNAM-1 on Melan-A sp. T cells (d + 10) with (empty) and without (tinted) IL12 pre-incubation. Dark-gray: isotype control. (B) Left: change of MFI over isotype in CD45 and subtype expression in STEAP1-sp. clonal populations incubated or not with 10 ng/mL IL12. Right: representative histograms, dark gray is isotype control, light gray no IL12 and black line IL12 incubation. (C) STEAP1 sp. clonal T cells were or were not pre-incubated with IL12 and tested against TC71 Ewing's sarcoma cell line. Reagents were present during assay as indicated (dosages as in 4(B), CD45-inhibitor 3.5 µM) after a pre-incubation time of 90 min on the T cells. Results are from three different experiments, spontaneous apoptosis is subtracted. E/T-ratio 20:1.