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. 2016 Mar 28;5(7):e1164918. doi: 10.1080/2162402X.2016.1164918

Figure 6.

Figure 6.

Myeloid PTEN deficiency induces regulatory T-cells. (A) Quantification of flow cytometry analysis of (live, CD45+ CD3+) CD4+ T-helper and CD8+ CTLs in the spleens of myPTEN+/+ and myPTEN−/− mice 10 d after s.c. B16 melanoma injection, values are expressed as % of live cells, n = 4–7; (B) Quantification of flow cytometry analysis of activation markers PD-1, CD25, CD69 and CD107a in CD4+ T-helper and CD8+ CTLs in the spleens of myPTEN+/+ and myPTEN−/− mice 10 d after s.c. B16 melanoma injection, values are expressed as % of CD4+ or CD8+, respectively, n = 8–14, *p < 0.05, **p < 0.01; (C) in vitro killing of B16-Ova-Luc cells was assayed by measurement of remaining luciferase activity specific for transduced melanoma cells after co-culture for 1 h with LPS/SIINFEKL-primed splenocytes from B16-injected myPTEN+/+ or myPTEN−/− mice as in (A) and (B), respectively, values represent arbitrary luminescence values; n = 2–7, *p < 0.05, **p < 0.01.