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. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: Cell. 2016 Apr 21;165(3):643–655. doi: 10.1016/j.cell.2016.03.045

Figure 4. Rigosertib Interferes with Growth Factor-Induced RAF Dimerization and MEK/ERK Activation.

Figure 4

(A–F) HeLa (A) or A431 (B) cells were serum starved for 18 hr in the presence of DMSO, RGS, or PLX, treated with EGF and cell lysates subjected to immunoprecipitation with anti-c-RAF antibodies and the level of associated B-RAF determined by western blot analysis. The level of ERK, phospho-ERK, MEK, phospho-MEK, total c-RAF and B-RAF, and GAPDH (loading control) were also determined by western blot analysis. The effect of DMSO, RGS, or PLX on EGF-induced RAF dimerization and/or activation of MEK and ERK in HeLa (C) cells transfected with a vector control or HA-tagged, N-RAS (G12D) expression vector HCT-116 (D) and A549 (E) and WM1617 (F) cells were determined as described above.

See also Figure S3.