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. 2016 Aug 31;11(8):e0161615. doi: 10.1371/journal.pone.0161615

Fig 3. Cell activity and cytoskeleton were maintained after each condition.

Fig 3

Cell activity was measured by resazurin assay (A-B) and cytoskeleton was visualized by f-actin staining (C-J) for NP and AF after each condition. Values are normalized to control without loading and represented in mean ± SEM. Control: Control discs without loading, HS: Heat-Shock, SL: Static Loading, DL: Dynamic loading, DL+Rest: Dynamic loading with Resting, DL*2: dynamic loading for two days, DL+Rest*2: dynamic loading and resting for two days. NP: Nuclues pulposus, AF: Annulus fibrosus. N = 5. RFU: relative fluorescence unit.