Fig 3. Leishmania Ag–stimulated cytokine profiles in splenocytes culture supernatants from naive, LdCen-/- immunized (Imm), naive challenged (Naive Chal), and LdCen-/- immunized challenged (Imm Chal) young and aged mice.
The 5-wk post immunized mice were challenged with virulent L. donovani for 4-wk and then mice were euthanized, splenocytes were isolated, plated aseptically (2×105 cells/well), and stimulated with Leishmania FTAg for 72h. Concentrations of pro-inflammatory cytokines IFN-γ (A), IL-12 (B) and TNF (C) anti-inflammatory cytokines IL-10 (D) and IL-4 (E) were measured in culture supernatants using the multiplex mouse cytokine kit as described in the Material and Methods section. Ratio of IFN-γ/IL-10 (F) and IFN-γ/IL-4 (G) were also determined. The data presented are representative of two independent experiments with similar results (n = 6). Mean and SEM of each group are shown. *p<0.05, **p < 0.005. Black bar indicates young mice and white bar indicates aged mice.