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. 2016 Aug 31;10(8):e0004963. doi: 10.1371/journal.pntd.0004963

Fig 7. Ag-specific intracellular cytokine secretion analysis of CD4 and CD8 T cells from LdCen-/- immunized and non-immunized young and aged mice after virulent L. donovani challenge.

Fig 7

The 8- wk post-immunized or non-immunized young and aged mice were challenged for 4-wk with virulent L. donovani. Intracellular cytokine analysis was done as shown in Fig 6A and divided into seven distinct subpopulations. (A) Cytokine analysis of CD4 T cells from 8-wk post immunized and 4-wk post challenged mice. (B) Cytokine analysis of CD8 T cells of 8-wk post immunized and 4-wk post challenged mice. (C) IL-10 secreting CD4 T cells and (D) the ratio of IFN-γ to IL-10 producing CD4 T cells from spleens at the time of challenge [(naive and immunized (8W)] and after challenge [(naive-challenged and immune-challenged (8WI plus 4WPC)]. The data presented are representative of two experiments with similar results. Mean and SEM of six mice in each group are shown. *p < 0.05, **p < 0.005. Black bar indicates young mice and white bar indicates aged mice.