Table 1.
Compound | LOD (ng/L) | LLOQ (ng/L) | ULOQ (ng/L) | Recovery (% ± 95% CI) | RSD (%) | Quantification Method |
---|---|---|---|---|---|---|
DOSS | 67* | 67 | 34,000 | 88 ± 10 | 10 | ISC: 13C4-DOSS |
α-/β-EHSS | 16† | 150† | 25000 | 98 ± 6 | 1.4 | ISC: 13C4-α-EHSS |
Span 80 | 1,250 | 3,000 | 60,000 | 91 ± 21 | 23 | Ext. Cal |
Tween 80 | 987 | 2,700 | 400,000 | 119 ± 13 | 10 | Ext. Cal |
Tween 85 | 99 | 700 | 150,000 | 106 ± 20 | 17 | Ext. Cal |
DOSS LOD is equal to DOSS LOQ due to background variability.
ISC: 13C4-DOSS - internal standard calibration using 13C4-DOSS as internal standard; ISC: 13C4-α-EHSS – internal standard calibration using 13C4-α-EHSS as an internal standard; and Ext. Cal. - external standard calibration