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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Microbiol. 2016 Jun 10;101(3):495–514. doi: 10.1111/mmi.13404

Fig. 8. CodY controls the secretion of thermonuclease in exponentially growing cells.

Fig. 8

A. Secreted thermonuclease activity in exponentially growing UAMS-1 cell culture supernatants was quantified using a FRET-based assay in strains producing varying amounts of residual CodY activity as described in Experimental Procedures. UAMS-1 (WT), SRB337; codY null mutant (None), SRB372; and the CodY variants R61K (SRB506), R61H (SRB508), R61E (SRB577) and G129D (SRB493). B. Secreted nuclease activity in a CodY-deficient strain relative to a SarA-deficient strain during exponential growth in TSB. nuc, UAMS-1471; UAMS-1, SRB337; codY, SRB372; sarA, UAMS-929. SarA has been shown previously to suppress Nuc enzyme levels (Kiedrowski et al., 2011) and was used as a positive control. Asterisks (*) denote statistical significance by Games-Howell pairwise test (p < 0.05) when compared to the codY null mutant. The data are presented as the mean ± the standard error of at least 2 independent experiments.