Table 1.
ilvD | hld | |||||
---|---|---|---|---|---|---|
Relevant genotype |
[ILV] (μM) |
doubling time (min) |
Transcript1 | % expression2 |
Transcript | % expression |
WT3 | 1,500 | 56 (3) | 0.03 (0.001) | 0 | 8.8 (1.9) | 0 |
150 | 53 (5) | 0.05 (0.006) | 0.5 | 11.5 (3.4) | 0.5 | |
15 | 120 (21) | 7.60 (1.40) | 173 | 81.9 (3.9) | 14 | |
1.5 | 99 (9) | 5.20 (0.90) | 118 | 60.1 (7.4) | 10 | |
codY | 1,500 | 66 (2) | 4.40 (0.37) | 100 | 518.9 (159.1) | 100 |
Transcript abundance normalized to polC abundance during growth in CDM containing the indicated concentration of ILV. Data are presented as the means (SEM) of at least two independent experiments.
% expression calculated using the equation: ((WT[ILV]-WT1500)/(codY1500/WT1500)) × 100.
UAMS-1