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. 2016 Sep 1;27(17):2642–2652. doi: 10.1091/mbc.E16-05-0290

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© 2016 Sung et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Overexpression of ribosomal proteins is inefficient. (A) Experimental scheme to evaluate overexpression of HHZ-tagged ribosomal proteins. (B) Steady-state levels of ribosomal proteins are lower than that of the nonribosomal protein Hog1 when all proteins are transiently overexpressed from the GAL promoter. Total cell lysates were fractionated by SDS–PAGE and immunoblotted with HA antibody. Hexokinase was used as an internal control. (C) Quantification of HHZ-tagged proteins in B. Relative HHZ-tagged protein levels were normalized to Hog1 using Odyssey software. Values are the mean of two independent experiments, and error bars indicate SDs.