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. 2016 Sep 1;27(17):2662–2674. doi: 10.1091/mbc.E16-05-0286

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© 2016 Cunniff, McKenzie, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Mitochondria traffic into the distal tips of 3D invadopodia in an AMPK-dependent manner. (A) SKOV-3, (B, C), SKOV-3ip, and (D) B16F10 cells expressing mTurquoise-LifeAct and mito-DsRed (yellow) were embedded in 3D collagen I gels overnight and then imaged over the indicated times. Scale bars, 10 μm (A, B, D), 20 μm (C). (E) Time course of mitochondrial dynamics in 3D collagen-embedded SKOV-3 cells expressing PA-AIP or PA-AIPscr before (Ctrl) and after photoactivation (+PA) by exposure to 445-nm blue light, as described for Figure 6 (bar, 5 μm). Multicolored images are temporal color-coded stack projections of mitochondrial movement over the indicated time points. (F) Quantification of mitochondrial velocities in the invading leading edges of SKOV-3 cells expressing PA-AIP or PA-AIPscr, embedded in 3D collagen gels, before and after photoactivation by 445-nm light (boxes, 25th–75th quartiles; whiskers, minimum and maximum; bars, mean ± SD; 30 measurements from three experiments; *p < 0.001).