Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 1;27(17):2688–2696. doi: 10.1091/mbc.E16-03-0196

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Maeda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 5: — TANGO1S forms a 700-kDa complex with cTAGE5 and Sec12, and TANGO1L forms a 900-kDa complex with cTAGE5 and Sec12. Two-dimensional Blue-Native PAGE/SDS–PAGE analysis of immunoprecipitants from an HSC-1 inducible cell line. (A) TANGO1S-FLAG or (B, C) cTAGE5-FLAG expression was induced by incubation with 10 ng/ml doxycycline for 24 h in HSC-1 stable cell lines. Cell proteins extracted with 1% digitonin were immunoprecipitated with anti-FLAG antibody and eluted with a FLAG peptide. Eluates were analyzed by two-dimensional Blue-Native PAGE/SDS–PAGE analysis, followed by Western blotting with (A, B) anti–TANGO1-CC1, anti-cTAGE5, and anti-Sec12 antibodies or (C) silver staining.