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. 2016 Sep 1;27(17):2688–2696. doi: 10.1091/mbc.E16-03-0196

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© 2016 Maeda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — cTAGE5 and TANGO1S are capable of forming homotrimers. 293T cells were transfected with (A) cTAGE5-FLAG, cTAGE5-HA, and cTAGE5-Myc constructs or (B) TANGO1S-FLAG, TANGO1S-HA, and TANGO1S-Myc constructs as indicated. Cell lysates were immunoprecipitated with anti-FLAG antibody and eluted with a FLAG peptide. Eluates were then immunoprecipitated with anti-HA antibody, and immunoprecipitants were subjected to SDS–PAGE, followed by Western blotting with anti-FLAG, anti-HA, or anti-Myc antibodies.