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. 2016 Sep 1;27(17):2708–2725. doi: 10.1091/mbc.E16-05-0337

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© 2016 Finnigan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Septin-binding domains of Bud4 and Hof1 have distinct subunit preferences. (A) Cells (strain GFY-42) expressing Cdc10-mCh and coexpressing Bud4(623-774)-eGFP under the control of the BUD4 promoter from a CEN plasmid (pGF-IVL1095) were grown and visualized as in Figure 4A. (B) Diploids (287–291) expressing Bud4(623-774)-(linker)₃₃-β11 from its native promoter on a LEU2-marked CEN plasmid (pGF-IVL1082), the β10-(linker)₁₈–tagged versions of each of the five mitotic septins (left), a URA3-marked CEN plasmid (pGF-IVL1005) expressing GFPβ1-9 from the GAL1/GAL10 promoter (unpublished data), and either Cdc10-mCh or Cdc11-mCh (as indicated) were selected on SD-Ura-Leu medium, grown, induced with galactose, washed, and imaged as in Figure 1B. (C) Quantification, as in Figure 1C, of the data in B. (D) Cells (strain GFY-42) expressing Cdc10-mCh and coexpressing eGFP-Hof1(293-355) under the control of the HOF1 promoter from a CEN plasmid (pGF-IVL1110) were grown and visualized as in Figure 4A. (E) Diploids (292–296) expressing Hof1(293-355)-(linker)₃₃-β11 from its native promoter on a LEU2-marked CEN plasmid (pGF-IVL1082), the β10-(linker)₁₈–tagged versions of each of the five mitotic septins (left), a URA3-marked CEN plasmid (pGF-IVL1005) expressing GFPβ1-9 from the GAL1/GAL10 promoter (unpublished data), and either Cdc10-mCh or Cdc11-mCh (as indicated) were treated and examined as in B. (F) Quantification, as in Figure 1C, of the data in E.