TABLE 1:
Primers sequences used in site-directed mutagenesis reactions.
| Primer name | Primer sequence |
|---|---|
| ERK1(Y280C)_F | 5′-GAGACTGTAGGCAGTTTCGGGCCTTCATG-3′ |
| ERK1(Y280C)_R | 5′-CATGAAGGCCCGAAACTGCCTACAGTCTC-3′ |
| ERK2(Y261C)_F | 5′-GCTAGAAACTGTTTGCTTTCTCTCCCGCAC-3′ |
| ERK2(Y261C)_R | 5′-GTGCGGGAGAGAAAGCAAACAGTTTCTAGC-3′ |
| ERK1(Y280A)_F | 5′-GAAGCCCCGAAACGCCCTACAGTCTCTGCCC-3′ |
| ERK1(Y280A)_R | 5′-GGGCAGAGACTGTAGGGCGTTTCGGGCCTTC-3′ |
| ERK2(Y261A)_F | 5′-GCTAGAAACGCTTTGCTTTCTCTCCCGCAC-3′ |
| ERK2(Y261A)_R | 5′-GTGCGGGAGAGAAAGCAAAGCGTTTCTAGC-3′ |
| MPK1(Y268A)_F | 5′-GGTTCTAAAAATGTTCAGGACGCCATACATCAATTAGG-3′ |
| MPK1(Y268A)_R | 5′-CCTAATTGATGAATGGCGTCCTGAACATTTTTAGAACC-3′ |
| MPK1(T190A+Y192F)_F | 5′- CAGTCAATTTTTGGCGGAGTTCGTGGCCACTAGATGG-3′ |
| MPK1(T190A+Y192F)_R | 5′-CCATCTAGTGGCCACGAACTCCGCCAAAAATTGACTG-3′ |
| MPK1(D326N)_F | 5′-CTATATGGCATGATCCAGCTAACGAACCTGTGTGTAGTG-3′ |
| MPK1(D326N)_R | 5′-CACTACACACAGGTTCGTTAGCTGGATCATGCCATATAG-3′ |
| MPK1(Y238F)_F | 5′-CTTCAAAGGAAAGGATTTCGTTAATCAATTGAATC-3′ |
| MPK1(Y238F)_R | 5′-GATTCAATTGATTAACGAAATCCTTTCCTTTGAAG-3′ |