Skip to main content
. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: Biochim Biophys Acta. 2011 Jul 5;1808(10):2359–2365. doi: 10.1016/j.bbamem.2011.06.016

Fig. 2.

Fig. 2

IB analysis of periplasmic proteins extracted from STEC and ETEC mutants. (A) The STx2A subunit was detected in the PPE samples of STEC O157 Sakai derivatives. STx2A band was present only in the wild type strain (lane 1) but absent in the both Δstx2B and Δstx2B/ΔdegP::Cm mutants (lanes 2 and 3, respectively). Inactivation of degP in the Δstx2B mutant background did not rescue the STx2A band in the periplasm (lane 3), suggesting that DegP might be not associated with the absence of STx2A. BAP (bacterial alkaline phosphatase as a periplasmic protein marker) was used as an internal control for the PPE samples. (B) PPE samples of ETEC H10407 and its LT-B-deficient mutant (ETEC/dLT-B) were analyzed by using anti-LT-A mAb (Abcam). As shown, the LT-A subunit (~28 kDa band) was absent in the Δlt-B mutant (lane 2), compared to wild type strain (lane 1).