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. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: Biochim Biophys Acta. 2011 Jul 5;1808(10):2359–2365. doi: 10.1016/j.bbamem.2011.06.016

Fig. 3.

Fig. 3

IB analysis of PPE samples of JM109 transformants. (A) PPE samples prepared from the JM109 cells harboring the respective pBAD-stx2A and pBAD-stx2AB clone were immunoblotted with anti-STx2A mAb. (B) For the complementation of JM109/pBAD-stx2A, plasmid pAY-stx1B or pAY-stx2B was introduced. STx2A band was strongly detected in the complementation with either pAY-stx2B or pAY-stx1B, whereas STx2A subunit overexpressed in the JM109/pBAD-stx2A strain (lane 1) was hardly detectable by IB with anti-STx2A mAb. (C) IB was performed to see whether A1 fragment of STx2A can exist alone in the periplasm of JM109. PPE of JM109/pBAD-stx2A1 contained the A1 fragment, albeit at reduced amount (lane 4) compared to the A1 fragment complemented with STx2B (lane 5).