Table 1.
Bacterial strains and plasmids used in this study.
| Strains/plasmids | Property | Source/Refs. |
|---|---|---|
| E. coli O157:H7 strain Sakai | Wild type | [8] |
| Sakai-DM | ΔmsbB1/msbB2 mutant of wild type Sakai strain | This study |
| DM/Δstx1B | Δstx1B mutant of Sakai-DM | This study |
| DM/Δstx1B/stx2B | Δstx1B/stx2B::Cm mutant of Sakai-DM | This study |
| DM/Δstx2A::Cm | Δstx2A::Cm mutant of Sakai-DM | [7] |
| WT/Δstx1B | Δstx1B mutant of Sakai wild type (WT) | This study |
| WT/Δstx2B | Δstx2B mutant of Sakai-WT | This study |
| WT/Δstx2B/ΔdegP | ΔdegP::Cm mutant of Sakai-WT/Δstx2B | This study |
| ETEC H10407 | A reference strain of enterotoxigenic E. coli (LT+) | Lab collection |
| ETEC/dLT-B | Δlt-B::Cm mutant of ETEC H10407 | This study |
| E. coli JM109 | endA1 gyrA96 thi hsdR17 supE44 relA1 traD36 Δ (lac-proAB)/F′(proAB lacIqZ M15) | Lab collection |
| pKD46 | A tS-plasmid encoding arabinose-inducible λ-Red recombinase (AmpR) | [18] |
| pKD3 | A template plasmid carrying the FRT-Cm-FRT cassette (CmR) | [18] |
| pCP20 | A tS-plasmid encoding the FLP recombinase (AmpR/CmR) | [18] |
| pSTx1B::Cm | pUC18-based recombinant plasmid carrying the Δstx1B::Cm allele (CmR/AmpR) | This study |
| pSTx2B::Cm | pUC18-based recombinant plasmid carrying the Δstx2B::Cm allele (CmR/AmpR) | This study |
| pDegP::Cm | pUC18-based recombinant plasmid carrying the ΔdegP::Cm allele (CmR/AmpR) | This study |
| pUC18 | General cloning vector (AmpR) | Lab collection |
| pACYC184 | General cloning vector (CmR), p15A (rep) origin | Lab collection |
| pAY-stx1B | pACYC184-based recombinant plasmid carrying the stx1B gene (CmR) | This study |
| pAY-stx2B | pACYC184-based recombinant plasmid carrying the stx2B gene (CmR) | This study |
| pBAD24 | An arabinose-inducible expression vector (AmpR), pBR322 (pMB1 rep) origin | [23] |
| pBAD-stx2A | pBAD24-based recombinant plasmid carrying the stx2A gene (AmpR) | This study |
| pBAD-stx2AB | pBAD24-based recombinant plasmid carrying the stx2AB operon (AmpR) | This study |
| pBAD-stx2A1 | pBAD24-based recombinant plasmid carrying the stx2A1 allele (AmpR) | This study |