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. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: FEBS Lett. 2006 Aug 10;580(20):4877–4883. doi: 10.1016/j.febslet.2006.07.086

Table 2.

Binding results, obtained with tryptophan quenching, of fatty acids, phospholipids and other molecules with Blc

Fatty acids Nr. carbons/chemical structure Kd (μM) Relative affinity
Myristic 14 graphic file with name nihms5900t1.jpg 3.3 ± 0.3 0.8
Palmitoleic 16 graphic file with name nihms5900t2.jpg 3.1 ± 0.2 0.9
Palmitic acid 16 graphic file with name nihms5900t3.jpg 2.7 ± 0.2 1
cis-Vaccenic 18 graphic file with name nihms5900t4.jpg 2.5 ± 0.3 1.1
Oleic 18 graphic file with name nihms5900t5.jpg 2.0 ± 0.1 1.35
Arachidonic 20 graphic file with name nihms5900t6.jpg 1.8 ± 0.1 1.5
Phospholipids
PtdGro 4.7 ± 0.3 0.57
E.coli total mix 2.5 ± 0.3 1.1
E.coli polar mix 2.9 ± 0.4 0.9
Lysophospholipids 1-Acyl-2-Hydroxy-sn-Glycero-3-Phosphoethanolamine
Oleoyl 0.039 ± 0.006 70
Palmitoyl 0.011 ± 0.001 245
Other molecules
Retinol graphic file with name nihms5900t7.jpg 20.2 ± 2 0.13
Ampicillin graphic file with name nihms5900t8.jpg 5.0 ± 0.3 0.54

Kd values are given in μM.