TABLE 1.
Bacterial strains and plasmids used in this study
| Strains/plasmids | Descriptiona | Source |
|---|---|---|
| E. coli O157:H7 | ||
| 4304 | Wild type (phage-type 14), (Strr) | Ref. 19 |
| 4304-DM | 4304 ΔmsbB1, ΔmsbB2 (Strr) [msbB1/2] | Ref. 19 |
| 4304-PM | 4304 pagP::aacC1 (Strr, Gmr) [pagP] | This study |
| 4304-TM | 4304 ΔmsbB1, ΔmsbB2, pagP::aacC1 (Strr, Gmr) [msbB1/2/pagP] | This study |
|
| ||
| E. coli K-12 | ||
| MC1061 | F-, λ-, araD139, Δ (ara-leu)7697, Δ (lac)X74, galU, galK, hsdR2 (rK-mK+), mcrB1, rpsL | Ref. 33 |
| WJ0124 | MC1061 pagP::amp [pagP] | Ref. 33 |
| SK1061 | MC1061 msbB::Tn5, pagP::amp [msbB/pagP] | This study |
| MC-msbB | MC1061 msbB::Tn5 [msbB] | Ref. 18 |
| BMS67C12 | msbB::Tn5 mutant of JM83 (Kmr) | Ref. 36 |
| SM10 | thr, leu, tonA, lacY, supE, recA::RP4-2-Tc::Mu, Kmr, λpir | Ref. 61 |
|
| ||
| Plasmids | ||
| pGEM-T | PCR product cloning vector | Promega |
| pUCGM | Gmr cassette (aacC1)-containing plasmid | Ref. 62 |
| pR7Crc-Gm | pRE107 carrying pagP::aacC1 | This study |
| pRE107 | A suicide vector (oriR6K, RP4 mob, sacB, Apr) | Ref. 61 |
| pCrcAT | pagPO157 in pGEM-T vector | This study |
| pWG24 | waaGO157 cloned into pBAD24 (AmpR) | This study |
| pGU184 | galUO157 cloned into pACYC184 (CmR) [pGalU] | This study |
| pAA101 | E. coli galETK cloned in pBR313 | Ref. 41 |
| pACPagP | E. coli pagP cloned in pACYC184 | Ref. 33 |
| pACPagPSer77Ala | Derivative of pACPagP | This study |
| pACPagPΔ5–14 | Previously pACPagPΔ30–39 (precursor numbering) | Ref. 33 |
| pWQ3 | K. pneumoniae rfbkp01 cloned in pRK404 | Ref. 48 |
| pBAD-B2 | E. coli msbB2 cloned in pBAD24 [pMsbB2] | Ref. 19 |
| pEP24 | pagPO157 in pBAD24 | This study |
a
Abbreviated descriptions used in some figures are included in square brackets.