TABLE 2.
Minimal inhibitory concentrations of antibiotics in E. coli lipid A acylation mutants
| Strains | Minimal inhibitory concentrationa
|
|
|---|---|---|
| Vancomycin | Novobiocin | |
|
| ||
| μg/ml | ||
| Wild-type O157:H7 4304 | >100 | >100 |
| pagP::aacC1 | >100 | 100 |
| ΔmsbB1, ΔmsbB2 | 50 | 50 |
| ΔmsbB1, ΔmsbB2, pagP::aacC1 | 25 | 3.125 |
| Wild-type K-12 MC1061 | >100 | >100 |
| pagP::amp | >100 | >100 |
| msbB::Tn5 | >100 | >100 |
| msbB::Tn5, pagP::amp | >100 | 100 |
a
Minimal inhibitory concentration was determined by a microdilution procedure using Mueller-Hinton broth as described under “Experimental Procedures.” The results are mean values of at least two independent experiments.