Fig. 8.

Detection of H₂O₂ from samples of Aβ, Cu²⁺, and/or a compound in the presence of ascorbate by a horseradish peroxidase (HRP)/Amplex Red assay. Lanes: (1) Aβ; (2) Aβ + Cu²⁺; (3) Aβ + Cu²⁺ + FL1; (4) Aβ + Cu²⁺ + FL2; (5) Aβ + Cu²⁺ + FL3; (6) Aβ + Cu²⁺ + FL4 ; (7) Aβ + Cu²⁺ + myricetin. Experimental conditions: [Aβ] = 200 nM, [Cu²⁺] = 400 nM, [ligand] = 800 nM, [ascorbate] = 10 μM, [Amplex Red] = 50 μM, [HRP] = 1 U/mL, λ_ex/em = 530/590 nm. Catalase attenuates the fluorescence signal.