Skip to main content
. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Nat Cell Biol. 2016 Aug 1;18(9):917–929. doi: 10.1038/ncb3394

Figure 2. Skeletal and SSC-Associated Defects in Snail/Slug-Targeted Mice.

Figure 2

(a) Femur histology of E17.5 Snailf/f/Slug+/+, Snailf/f/Slug+/+/Dermo1-Cre Snailf/f/Slug−/− and Snailf/f/Slug−/−/Dermo1-Cre embryos. Scale bar: 1 mm. Results are representative of 5 experiments performed.

(b) Alizarin Red/Alcian Blue staining of skulls isolated from E17.5 Snailf/f/Slug+/+, Snailf/f/Slug+/+/Dermo1-Cre, Snailf/f/Slug−/− and Snailf/f/Slug−/−/Dermo1-Cre embryos. Scale bar: 500 μm. Results are representative of 5 experiments performed.

(c) Histology of E15 Snailf/f/Slug+/+ and Snailf/f/Slug+/+/Dermo1-Cre skulls across the parietal area. Scale bar: 100 μm. Results are representative of 5 experiments performed.

(d) Relative thickness of skull mesenchymal cell layers from (c) (mean ± s.d., n=5 mice). **p<0.01; unpaired t-test.

(e) Ki67 Immunofluorescence of parietal suture mesenchymal cell layers from E15 Snailf/f/Slug+/+ and Snailf/f/Slug−/−/Dermo1-Cre skulls. Scale bar: 25 μm. Results are representative of 4 experiments performed.

(f) Quantification of Ki67-positive cells from (e) (mean ± s.d., n=4 mice). **p<0.01; unpaired t-test.

(g) Relative mRNA expression of osteogenic markers in E17.5 calvarial RNA extracts from Snailf/f/Slug+/+ versus Snailf/f/Slug−/−/Dermo1-Cre embryos (mean ± s.d., n=5 mice). **p<0.01; unpaired t-test.

(h) CFU-F generation from bone marrow cells isolated from E18.5 Snailf/f/Slug+/+, Snailf/f/Slug+/+/Dermo1-Cre, Snailf/f/Slug−/− and Snailf/f/Slug−/−/Dermo1-Cre mice. Results are representative of 5 experiments performed.

(i) CFU-F colony counts from (h) (mean ± s.d., n=5 mice). **p<0.01; one-way ANOVA.

(j) Schematic of the overall experimental design for in vivo implantation (upper panels).The lower panels show histology of tissues isolated from nude mice transplanted with Snailf/f/Slug+/+/GFP or Snailf/f/Slug−/−/Cre SSCs. Results are representative of 3 performed. Scale bar: 100 μm.

(k) Quantification bone formation in tissues from (j) (mean ± s.d., n=3 mice). **p<0.01, *p<0.05; one-way ANOVA.