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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Pharm Res. 2016 Jun 28;33(10):2540–2551. doi: 10.1007/s11095-016-1978-1

Figure 4. Liposome penetration in spheroids.

Figure 4

Figure 4

(a) Z-stack images taken by confocal microscopy show penetration of untargeted and Tf-targeted rhodamine (Rh)-labeled liposomes in 4 days old NCI/ADR-RES spheroids. The images are of spheroid sections at 0, 33, 77 and 99 μm distance from the periphery. The red color indicates the presence of Rh-labeled liposomes. The association with the deeper layers of the spheroid was higher with Tf-targeted liposomes. (b) The corrected rhodamine fluorescence intensity vs distance from the periphery quantitates the liposome association of targeted and untargeted liposomes in NCI/ADR-RES spheroids.