Fig. 5.

The effect of belinostat on cell cycle distribution of LN-229 and LN-18 glioblastoma cell lines. The cell cycle was measured by propidium iodide staining followed by flow cytometry analysis. Results are shown for cells treated with 0.5 and 2 μmol/L Bel for 24 and 48 h versus untreated controls. Graphical representation of the cell cycle profiles obtained from flow cytometry measurements in LN-229 (a) and LN-18 cells (b) are shown. Bar graphs presenting the percentage of cell cycle distribution in LN-229 cells after 24 h and 48 h (c); LN-18 cells after 24 h and 48 h (d) of treatment with belinostat. Significant alterations are expressed relative to controls and marked with asterisks. Statistical significance was considered if * p < 0.05