Fig. 3.

Loss of HNF4α leads to increased proliferation in the gastric epithelium. A: immunofluorescent staining with Ki67 (green) shows increased proliferation in ΔHNF4α vs. control mouse gastric corpus (mucous neck cells labeled red with GS-II lectin). Note orientation of gastric units with gastric lumen to left and base to right; A and B focus only on isthmus and neck zones. B and C: staining and quantification of BrdU (green) incorporation in control and ΔHNF4α epithelium. Columns = means ± SE, n = 3 biological replicates; ***P < 0.001. D: increased proliferation correlated with longer units. n = 4 mice; mean ± SE; *P < 0.05. Scale bars = 20 μm.