Fig. 6.

Zymogenic cell abnormalities in the absence of HNF4α are caused by loss of HNF4α targets regulating ER and the cell cycle. Immunofluorescent stain of the ER marker Calregulin (red) and the mucous neck cell marker GSII (magenta) in control and ΔHNF4α mice (nuclei: blue). Note less elaborate ER network in HNF4α mice (left and right panels at same magnification). A: optical sections. B: higher magnification Z-stack 3-dimensional projection of 20 0.5-μm optical sections in different mice. C: optical section of ΔHNF4α showing occasional bases harboring small ZCs containing markedly scant ER. Representative ZCs outlined in dashed yellow with blue lines showing basement membrane surface. All scale bars = 10 μm.