Figure 1. Overview of the genome-editing methods based on TECCDNA or TECCRNA.

The CRISPR/Cas9 DNA (plasmid constructs) or RNA (in vitro synthesized transcripts) is delivered into immature wheat embryos by particle bombardment. After transient expression and function, CRISPR/Cas9 DNA or RNA becomes degraded, while the bombarded embryos produce callus cells from which seedlings are regenerated. The T0 seedlings are examined using PCR-RE assay and DNA sequencing to identify targeted mutants.