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Validation of expression profile of vinculin (VCL) in HF subjects. PBMC protein lysates (5 µg) from NH controls (n = 12) and HF subjects (n = 22) were subjected to sandwich and biotin-switch ELISA, respectively, for the detection of VCL (a) and SNO-modified VCL (b) levels. Mann Whitney U test was performed to evaluate the significance (∗∗ p < 0.01, ∗∗∗ p < 0.001).
