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(a–c) HH5 quail (brown) #3-mesoderm co-cultured in a chick host with cell pellets transfected with NRP1+FBLN7+KIRREL3+VTN (red) either overnight (a,b) or for 6–9 h (c). VMHC1/MYH15 and NPPB are induced in #3-mesoderm (a,b, red arrowheads) but MYOCD is not (c, black arrows). Control pellets (red; pCAB) do not induce VMHC1/MYH15, NPPB or MYOCD (a–c, black arrows). Scale bars, 0.5 mm in whole-mounts and 0.1 mm in insets and sections.
