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. 2016 Aug 25;7:12638. doi: 10.1038/ncomms12638

Figure 3. RNF168 mouse cells and human breast cancer cell lines to cytotoxic effects of TOP2 catalytic inhibitors and poisons.

Figure 3

(a–d) Sensitivity of WT and Rnf168−/− MEFs to ICRF-193 (a,b) or etoposide (c,d) was determined using clonogenic assays. (e,f) Sensitivity of the human breast cancer cell lines T47D and MDA-MB-231 to etoposide was determined using clonogenic assays. (a,c,e) Data are presented as the mean±s.e.m. (a,c, n>4; e, n=4). *P<0.05 for Rnf168−/− MEFs compared with WT MEFs and T47D and MDA-MB-231 cells knocked down for RNF168 (sh.RNF168) compared with their respective controls (Sh.Ctr: ShRNA control). (b,d) Representative pictures of dishes showing surviving WT and Rnf168−/− colonies post ICRF-193 (b) or etoposide (d) treatment. (f) Representative pictures of dishes showing surviving colonies of etoposide treated T47D and MDA-MB-231 cells.