Subject area	Male fertility diagnostics research
More specific subject area	Chicken (Gallus gallus) male gamete peptide/protein repository
Type of data	Figures, table
How data was acquired	Experiments performed on a LTQ orbitrap Velos Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to an Ultimate®3000 RSLC Ultra High Pressure Liquid Chromatographer (Dionex, Amsterdam, The Netherlands)
Data format	Processed, analyzed
Experimental factors	Sample consisted of subfertile chicken sperm
Experimental features	Protein were extracted from chicken sperm and subjected to chromatographic fractionation prior to identification using Top-Down High Resolution Mass Spectrometry. Results were subjected to a functional analysis using bioinformatics tools.
Data source location	Nouzilly, Indre-et-Loire, France
Data accessibility	Data is within this article and accessible via the PRIDE partner repository at https://www.ebi.ac.uk/pride/archive/projects/PXD002768