Fig. 1.

Ca²⁺ depletion causes disruption of epithelia barrier. Influence of Ca²⁺ deprivation on junctional protein expression (A, B) and function (C). NRK-E52 cells were exposure to Ca²⁺-free medium for the indicated time and subjected to immunofluorescence for ZO-1 (A), Western blot analysis for pan-cadherin (B) and TER measurement (C). β-actin in B was used as a loading control. Data shown in C are mean ± SE (n=5;* P<0.01 vs. control).