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. 2016 Sep 1;6:32104. doi: 10.1038/srep32104

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Strains harbouring the plasmids pET28b(pezTΔC₂₄₂(D66T)), pET28b(pezA/pezT), or pET28b(pezTΔC₂₄₂) produced (a) an inactivated toxin, (b) the antitoxin-toxin complex or (c) the C-terminal truncated toxin. Micrographs showing an E. coli colony with (d) inactivated toxin expression, (e) a colony of cells before and after expression of the antitoxin-toxin module, and (f) a colony expressing the truncated toxin, which caused cell lysis, are presented. Cells stained prior to IPTG induction (2.6 h) or prior to lysis are indicated with a yellow arrow. A white arrow indicates a cell that resisted toxin expression but did not resuscitate following a backshift to growth medium.