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. 2016 Sep 1;6:32537. doi: 10.1038/srep32537

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Caterpillars (left) and adults (right) of M. loreyi (OS_MYL) and P. guttata used in experiments. (b) FAC composition in oral secretions from M. loreyi (OS_MYL) and P. guttata (OS_PAG) determined by LC-MS operating in negative scan MS mode (m/z range 150–1000); typical extracted ion chromatograms (EICs) of similarly diluted OS_MYL and OS_PAG samples are shown at unified y-axes scales for direct comparison. (c) The FACs in individual isolates of OS_MYL and OS_PAG were determined as above and total FAC concentration in each batch of OS were estimated by pooling individual FAC peaks, and using synthetic FAC, N-linolenoyl-L-Gln as calibration standard. “nd” refers to samples with no FAC detected. Data (n = 3) are shown as means ± SD.