Figure 3. OS from M. loreyi and P. guttata elicits defense metabolic responses in rice cells.

Freshly prepared aliquots of rice cells in 1 mL culture media were treated with 2 μL OS from M. loreyi (OS_MYL) or P. guttata (OS_PAG) for 24 h (i.e., final 500-fold OS dilution). Treatment with fungal elicitor (GlcNAc)₈ at 10 nM concentration was included as positive control in all experiments. Metabolite accumulation (a) and gene expression levels (b) were determined 24 h and 1 h after elicitation, respectively. Data (n = 3–4) are shown as means ± SD, and asterisks show statistically significant differences between individual elicitor treatments and control (water) analyzed by Dunnett’s test (*P < 0.05).