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. 2016 Sep 1;6:32537. doi: 10.1038/srep32537

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Rice cells were treated with OS as before, and accumulation of ROS in culture media was determined by chemiluminescence assays with L-012 as substrate. (a) ROS accumulations captured 120 min after OS application (each OS_MYL and OS_PAG belong to independent batch of larvae #1–3). (b,c) Time resolved course of ROS accumulation elicited by crude OS (500-fold dilution) or OS dialysate (>3.5 kDa, 500-fold dilution), carefully adjusted to comparable amounts in both experiments. Data (n = 3) are shown as means ± SD, and asterisks show statistically significant differences between treatments and control determined by Dunnett’s test (*P < 0.05).