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. 2016 Sep 1;6:32537. doi: 10.1038/srep32537

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Rice cells were treated with synthetic N-linolenoyl-L-Gln (50 μM), linolenic acid (50 μM), Gln (50 μM), Tween-20 [0.01% (v/v)], OS_MYL (500-fold dilution), or (GlcNAc)₈ (10 nM). Stock solutions of N-linolenoyl-L-Gln, linolenic acid, and Gln were dissolved in 0.01% (v/v) Tween-20 that was included as one of negative control treatments. (a) Metabolite accumulation measured 24 h after elicitation. (b) ROS amounts captured 120 min after elicitation. (c) Time resolved ROS release 0–180 min after FAC and (GlcNAc)₈ treatments. Data (n = 3) are shown as means ± SD, and statistical differences were analyzed by ANOVA followed by Tukey HSD test (P < 0.05).