Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 1;6:32534. doi: 10.1038/srep32534

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Schematic representation of CsAN1 promoter. Red box indicate CpG island. (B) DNA methylation status of bisulfite-sequenced CpG island in five tea cultivars and (C) ‘ZJ’ leaf at four developmental stages. CG, CHG and CHH refer to the three different contexts of cytosines, in which H represents nucleotide A, C or T. Each data point represents a mean ± SD of three independent gDNA extractions with three independent technical replicates. Five independent clones from each reaction were sequenced and analyzed. (D) Real-time reverse transcription-PCR analyses of CsAN1 and anthocyanin biosynthesis related genes expression in ‘YH9’, ‘RHBH’ and ‘ZJ’ leaves treated with (+) or without (−) 5-aza-dC, an inhibitor of DNA methylation. Tea Actin was used as a control. Values are means ± SD of three biological replicates. Asterisks indicate significant differences (p < 0.01) by Student’s t-test.