Table 1. Effects of defensins on melting temperatures (Tm, °C) of viral and host proteins calculated from DSF.
| Protein alone | + RC-101 | + HNP1 | + HD5 | + hBD2 | |
|---|---|---|---|---|---|
| HIV-1 Gag-Δp6 | 38.6 ± 0.3 | n/a | n/a | 34.3 ± 0.6 | 38.9 ± 0.2 |
| HIV-1 CA | 51.9 ± 0.1 | 41 ± 0.1 | 44.2 ± 0.5 | 47.6 ± 0.2 | 51.4 ± 0.2 |
| HIV-1 RTp51 | 44.4 ± 0.1 | 37 ± 0.2 | 40.3 ± 0.1 | n/d | n/d |
| HIV-1 IN | 42.5 ± 0.5 | 38.3 ± 0.6 | 41.0 ± 0.3 | 42.0 ± 0.5 | 43.2 ± 0.9 |
| PFV IN | 47.6 ± 0.1 | 42.5 ± 0.1 | 43.3 ± 0.1 | 45.3 ± 0.1 | 46.5 ± 0.2 |
| TEV Protease | 43.7 ± 0.2 | 33.8 ± 1.8 | 34.9 ± 0.2 | 44.7 ± 0.2 | 44.0 ± 0.2 |
| PLS3 | 47.8 ± 0.1 | 45.7 ± 0.1 | 46.8 ± 0.2 | 47.5 ± 0.1 | 47.4 ± 0.1 |
| IgG | 63.8 ± 0.2 | 63 ± 0.1 | 64.5 ± 0.1 | 65.2 ± 0.2 | 64 ± 0.2 |
n/a – Not applicable (DSF curve does not have a peak).
n/d – Not determined.