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. 2016 Sep 1;6:32323. doi: 10.1038/srep32323

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The extracellular calcium deposition was stained by Alizarin Red S (A) Mg-1Ca-0.2Sr alloy, (B) Mg-1Ca-0.5Sr alloy, (C) Mg-1Ca-1.0Sr alloy, (D) Mg-1Ca-2.0Sr alloy, (E) Control group) and quantified using a colorimetric method (F) after osteoinduction for 14 days. Cells cultured in osteogenic medium were set as the control group. With the increase of Sr content in four alloys, extracellular matrix mineralization nodules were stained intensively and increased visibly. All data represent the mean ± standard deviation of three independent experiments. ^#p < 0.05 and ^##p < 0.01 compared with the blank control group.