Table 3. Primers sequence used for Real-time PCR.

Gene	Primer sequence
	Forward	Reverse
RUNX2	5′-TCCAACCCACGAATGCACTA-3′	5′-GAAGGGTCCACTCTGGCTTTG-3′
OSX	5′-AGAAGCCATACGCTGACCTTTC-3′	5′-AGTGAGGGAAGGGTGGGTAGTC-3′
Integrinα5	5′-AGCAACTGCACCTCCAACTACA-3′	5′-ACACTTGGCTTCAGGGCATT-3′
Integrinβ1	5′-TTGGTCAGCAACGCATATCTG-3′	5′-CAGCAAAGTGAAACCCAGCAT-3′
ALP	5′-GTCATCATGTTCCTGGGAGA-3′	5′-GGCCCAGCGCAGGAT-3′
BSP	5′-AATGGCCTGTGCTTTCTCGAT-3′	5′-CCCTGGACTGGAAACCGTTT-3′
COL I	5′-GCAGGGTTCCAACGATGTTG-3′	5′-AGGAACGGCAGGCGAGAT-3′
OCN	5′-CAATAAGGTAGTGAACAGAC-3′	5′-CTTCAAGCCATACTGGTCT-3′
OPN	5′-TCCAAAGCCAGCCTGGAAC-3′	5′-TGACCTCAGAAGATGAACTC-3′
GAPDH	5′-CATGGCCTTCCGTGTTCCTA-3′	5′-CCTGCTTCACCACCTTCTTGAT-3′