Figure 5. Despite lower glucose levels, plasma leptin concentration remains low and Myo^Ln/Ln diabetic mice do not show significant evidence of “beiging” iWAT stores.

For all graphs two-way ANOVA with Bonferroni post-hoc test was performed. *Indicates p < 0.05. White bar – diabetic; black bar – non-diabetic. (A) Plasma leptin measures at 8 weeks of diabetes. N = 3–4. (B) Leptin measurement normalized to the mass of the epididymal fat mass N = 3–4. (C) Epididymal fat mass, N = 7–12. (D) Area of iWAT lipid droplets. N  = 4–6, male and female. (E) Object area fraction of UCP-1 stain in iWAT tissue. N = 4–8, male and female.