Figure 2. Double flash-isolated cone responses from Nckx2^−/− and littermate control mice.

(A) Double flash method to isolate cone light responses in ex vivo retina preparation. Pre-flash delivering 12,000 photons (530 nm) μm⁻² (left green arrow) was followed by a test flash (right green arrow) delivered while the rods were still saturated. Inter-stimulus interval Δt was 200 ms. Inset: representative cone response family to test flashes ranging from 565–732,000 photons μm⁻². (B) Population averaged dim flash responses (I = 1,600 photons μm⁻²) and saturated bright flash responses (I = 183,000 photons μm⁻²) normalized with R_max in control (black, n = 6) and Nckx2^−/− (red, n = 8) retinas, respectively. The inset shows normalized dim flash responses from control (black) and Nckx2^−/− (red) retinas. Error bars show SEM.