Figure 2. Localisation of the Purkinje cells using histological findings and its extension into the third dimension.

(a) 3D view of Purkinje cells with a tomogram slice resembling a 3D extension of histology and (b–g) their identification. (b) Registered phase contrast image. (c) Phase contrast image coloured similar to H&E staining. (d) Purkinje cell segmentation mask. (e) Phase contrast image with separately coloured Purkinje cells to resemble f, H&E histology. The staining is insufficient to visualise the nucleolus, due to post mortem autolysis. g, Biospy - H&E section with nucleoli visible. The scale bar corresponds to 100 µm (see also Supplementary Video 2).