Figure 3. FACS purification of FolR1 labelled cells.
(A) A representative flow cytometry profile for FolR1-labelled cells generated according to a dopaminergic (left) or non-dopaminergic differentiation protocol (middle), and an unstained control of cells differentiated according to a dopaminergic protocol (right); (B) Flow cytometry analysis of FolR1 antibody-labelled Pitx3-GFP reporter cells differentiated according to a dopminergic protocol. Left: scatter plot of the cells prior to FACS sorting, Middle: scatter plot of the cells straight after sorting, Right: a histogramme showing Pitx3-GFP expression profile by FACS sorted FolR1+ cells. All analysis were performed at day 13 of differentiation.