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. 2016 Sep 1;6:32488. doi: 10.1038/srep32488

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Copyright © 2016, The Author(s)

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Cells were sorted on day 13 and cultured for 1 and 7 days after sorting. (A) 1 day cultured cells stained for ventral midbrain markers; (B) 7 day cultured cells stained for ventral midbrain markers; (C) 7 day cultured cells stained for GABA (GABAeric), 5HT (serotonergic) neuron and GFAP (astrocytes); (D) BrdU incorporation of sorted FolR1⁺ cells. Cells were sorted on day 10 of in vitro differentiation and subsequently cultured either in maturation media (+GDNF, BDNF) or in proliferation-promoting media (+SHH, +FGF8). BrdU was added for 3 hours before fixation at day 1 and day 7 post-sort, respectively.