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. 2016 Sep 1;6:32087. doi: 10.1038/srep32087

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Flow cytometric analysis of podocyte apoptosis with or without CaN siRNA after PAN treatment (*P < 0.05). (B) Podocyte mitochondria was visualized using MitoTracker Red CMXRos. Images were taken with confocal microscope. (C) Western blot analysis of cytochrome c (cytosolic fraction) with or without CaN siRNA after PAN treatment. (D) Fluorescent staining and flow cytometric analysis (E) of the effects of CaN siRNA on PAN-induced MMP in podocytes (*P < 0.05). All experiments were performed for 3 times, and the results were shown as mean ± SD.