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. 2016 Sep 1;6:32087. doi: 10.1038/srep32087

Figure 4. CsA and FK506 pretreatment protect against PAN-induced injury in cultured mouse podocytes in vitro.

Figure 4

(A) Immunofluorescence of F-actin, synaptopodin and podocin in PAN treated mouse podocytes after CsA and FK506 treatment. Original magnification, X400. (B) Western blot analyses of podocin and synaptopodin in PAN injured mousepodocytes after CsA and FK506 treatment for 24 h (podocin: N 0.49 ± 0.05, PAN 0.4 ± 0.06, PAN + CsA 0.6 ± 0.1, PAN + FK506 0.76 ± 0.14; synaptopodin: N 0.89 ± 0.03, PAN 0.52 ± 0.1, PAN + CsA 0.67 ± 0.09, PAN + FK506 0.9 ± 0.15; n = 3; *P < 0.05, **P < 0.01). All experiments were performed for 3 times, and the results were shown as mean ± SD.